| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fatty acid-binding protein, intestinal;Fatty acid-binding protein 2;Intestinal-type fatty acid-binding protein;I-FABP;FABP2;FABPI; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human FABP2/I-FABP, different from the related mouse sequence by seven amino acids, and from the related rat sequence by six amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects fatty acid-binding protein, intestinal (gene FABP2) in human, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 15.2 kDa, and the supplier reports an observed band at about 15 kDa.
Intestinal fatty acid-binding protein (FABP2, I-FABP) is a small cytosolic protein in enterocytes of the small intestine. It binds long-chain fatty acids and is thought to support their uptake and packaging into triglyceride-rich lipoproteins. Because it leaks into blood when the gut lining is damaged, I-FABP is widely measured as a marker of intestinal epithelial injury.
| Target | Fatty acid-binding protein, intestinal (gene FABP2; UniProt P12104, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 15.2 kDa |
| Observed MW | 15 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue, human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Synthetic peptide from the N-terminal region of human FABP2/I-FABP. It differs from the mouse sequence at seven residues and from the rat sequence at six residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places fatty acid-binding protein, intestinal in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in the small intestine and at much lower levels in the large intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ben-Haim Moshe Shay et al. (2021) Dynamic regulation of N6,2′-O-dimethyladenosine (m6Am) in obesity. Nature Communications. 10.1038/s41467-021-27421-2
X. W. Chen et al. (2012) Identification and expression analysis of fabp2 gene from common carp Cyprinus carpio. Journal of Fish Biology. 10.1111/j.1095-8649.2011.03203.x
