| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C motif chemokine 10;10 kDa interferon gamma-induced protein;Gamma-IP10;IP-10;Small-inducible cytokine B10;CXCL10 (1-73);CXCL10;INP10, SCYB10; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human IP10 recombinant protein (Position: V22-P98). Human IP10 shares 70.1% and 72.7% amino acid (aa) sequence identity with mouse and rat IP10, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects C-X-C motif chemokine 10 (gene CXCL10) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 10.9 kDa, and the supplier reports an observed band at about 34 kDa.
CXCL10 (IP-10) is an interferon-γ-inducible chemokine that binds the receptor CXCR3. It attracts activated T cells, NK cells and monocytes to sites of infection and inflammation and also has angiostatic effects. CXCL10 is commonly measured as a marker of interferon-driven immune responses in viral infection, autoimmunity and transplant research.
| Target | C-X-C motif chemokine 10 (gene CXCL10; UniProt P02778, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 10.9 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human IP10 fragment (Val22–Pro98), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 70.1% and 72.7%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes C-X-C motif chemokine 10 as a secreted protein. Tissue expression noted by UniProt (human): Mainly secreted by monocytes, endothelial cells as well as fibroblasts. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
