| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-X-C motif chemokine 10;10 kDa interferon gamma-induced protein;Gamma-IP10;IP-10;Small-inducible cytokine B10;CXCL10 (1-73);CXCL10;INP10, SCYB10; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human IP10. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-IP10/CXCL10 Antibody Picoband® is an antibody targeting CXCL10. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human; observed MW: 40 kDa; calculated MW: 10881 MW.
Boster Bio Anti-IP10/CXCL10 Antibody catalog # PA1396. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: CXCL10 — C-X-C motif chemokine 10
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 40 kDa; Calculated: 10881 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
Scientific background (datasheet): C-X-C motif chemokine 10 (CXCL10), also known as Interferon gamma-induced protein 10 kDa (IP-10) or small-inducible cytokine B10, is a protein that in humans is encoded by the CXCL10 gene. It is a small cytokine belonging to the CXC chemokine family. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. Luster et al. (1987) reported the isolation of an interferon-inducible gene that encodes a 98-amino acid protein called IP10. The chemokine IFN-gamma-inducible protein 10 (IP-10; CXCL10), a CXC chemokine, and its receptor, CXCR3, were found to be overexpressed in lymph node cells of EAMG rats. IP10 inhibits bone marrow colony formation, has antitumor activity in vivo, is a chemoattractant for human monocytes and T cells, and promotes T cell adhesion to endothelial cells.
Cellular localization (datasheet): Secreted.
Tissue details (datasheet): Expressed on activated B-cells.
Sequence similarities (datasheet): Belongs to the intercrine alpha (chemokine CxC) family.
Research relevance and current trends
- Commonly studied in contexts related to Angiogenesis,Cancer,Chemokines,Immunology,Innate Immunity,Invasion/Microenvironment.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
