| Field | Specification |
|---|---|
| Target | |
| Alternative names | tRNA dimethylallyltransferase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; hGRO1; tRNA isopentenyltransferase 1; IPTase; TRIT1; IPT; MOD5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human IPT/TRIT1 recombinant protein (Position: L16-K415). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tRNA dimethylallyltransferase (gene TRIT1) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 52.7 kDa, and the supplier reports an observed band at about 53 kDa.
TRIT1 is a tRNA isopentenyltransferase that adds a dimethylallyl group to adenosine 37, next to the anticodon, forming i6A37 in both cytosolic and mitochondrial tRNAs. Only a small group of tRNAs is modified, including the selenocysteine tRNA, so TRIT1 is needed for efficient selenoprotein production and for accurate mitochondrial translation.
| Target | TRNA dimethylallyltransferase (gene TRIT1; UniProt Q9H3H1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 52.7 kDa |
| Observed MW | 53 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human Raji cells, human K562 cells, human RT4 cells, human HEL cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human IPT/TRIT1 fragment (Leu16–Lys415), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
