| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-1 receptor-associated kinase 1; IRAK-1; IRAK1; IRAK |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IRAK |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABII-9 is a rabbit monoclonal antibody that recognizes interleukin-1 receptor-associated kinase 1 (gene IRAK1) in human samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 76.5 kDa, and the supplier reports an observed band at about 77 kDa.
IRAK1 (interleukin-1 receptor-associated kinase 1) is a serine/threonine kinase recruited to MyD88 after Toll-like receptor or IL-1 receptor activation. Once phosphorylated by IRAK4, it binds TRAF6 to drive NF-κB and MAPK signaling, so it is a key node in innate immune and inflammatory responses.
| Target | Interleukin-1 receptor-associated kinase 1 (gene IRAK1; UniProt P51617, human) |
|---|---|
| Clone | ABII-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 76.5 kDa |
| Observed MW | 77 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human IRAK.
Reactivity Notes
The supplier lists reactivity with human. UniProt places interleukin-1 receptor-associated kinase 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
