| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Bcl-2-like protein 11;Bcl2-L-11;Bcl2-interacting mediator of cell death;BCL2L11;BIM; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human IRAK2 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-IRAK2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 19I19; isotype IgG; reactivity: Human. Reported application contexts include WB, IP, Flow (as provided in the source record). Boster Bio Anti-IRAK2 Rabbit Monoclonal Antibody catalog # M01559-1. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: IRAK2 (Bcl-2-like protein 11).
- Antibody format: Monoclonal; clone 19I19; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
IRAK2 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis. . Reported cellular localization context: Endomembrane system ; Peripheral membrane protein . Associated with intracytoplasmic membranes. . Tissue expression notes (as provided): Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. .
Research relevance and current trends
- Research context keywords from the source record include: Alzheimer's Disease,Apoptosis,Apoptotic Markers,Associated Proteins,Cancer,Cell Biology,Cell Death,Immunology,Innate Immunity,Intracellular,Invasion/Microenvironment,Macrophage/Inflammation,MAPK Pathway,Metabolism,Metabolism Processes,Neurodegenerative Disease,Neurology Process,Neuroscience,Oncoproteins,Oncoproteins/Suppressors,Pathways and Processes,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate IRAK2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect IRAK2 expression by Western blot in cell or tissue lysates, Quantify IRAK2-positive cells by flow cytometry in single-cell suspensions, Enrich IRAK2 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 69 kDa; calculated MW: 22171 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 69 kDa
- Cellular localization (provided): Endomembrane system ; Peripheral membrane protein . Associated with intracytoplasmic membranes. .
- Tissue details (provided): Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
