| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon regulatory factor 1;IRF-1;IRF1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IRF1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DHE-9 is a rabbit monoclonal antibody that recognizes interferon regulatory factor 1 (gene IRF1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 36.5 kDa, and the supplier reports an observed band at about 48 kDa.
Interferon regulatory factor 1 (IRF1) is a transcription factor induced by interferons and other stimuli. It activates genes for type I interferons, interferon-stimulated genes and MHC class I, and it also regulates cell growth and apoptosis, so it is studied in antiviral immunity, inflammation and tumor suppression.
| Target | Interferon regulatory factor 1 (gene IRF1; UniProt P10914, human) |
|---|---|
| Clone | DHE-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 36.5 kDa |
| Observed MW | 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:1000 |
|---|---|
| Immunohistochemistry | 1:50–1:100 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:100 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with confirmed staining (IHC): rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human IRF1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places interferon regulatory factor 1 in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
