| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon regulatory factor 5;IRF-5;IRF5; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IRF5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GHI-9 is a rabbit monoclonal antibody that recognizes interferon regulatory factor 5 (gene IRF5) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 56.0 kDa, and the supplier reports an observed band at about 56 kDa.
Interferon regulatory factor 5 (IRF5) is a transcription factor activated downstream of endosomal Toll-like receptors TLR7, TLR8 and TLR9 via MyD88. It induces type I interferons and inflammatory cytokines and promotes inflammatory macrophage polarization, and genetic variants of IRF5 are associated with systemic lupus erythematosus.
| Target | Interferon regulatory factor 5 (gene IRF5; UniProt Q13568, human) |
|---|---|
| Clone | GHI-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 56.0 kDa |
| Observed MW | 56 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:1000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:100–1:500 |
| Immunoprecipitation | 1:100 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human IRF5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places interferon regulatory factor 5 in the cytoplasm and nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
