| Field | Specification |
|---|---|
| Target | |
| Alternative names | Insulin receptor substrate 1;IRS-1;IRS1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IRS1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GIF-9 is a rabbit monoclonal antibody that recognizes insulin receptor substrate 1 (gene IRS1) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 131.6 kDa, and the supplier reports an observed band at about 170 kDa.
Insulin receptor substrate 1 (IRS1) is an adaptor protein phosphorylated on tyrosine by the insulin and IGF-1 receptors. Phosphorylated IRS1 recruits PI3K and GRB2, relaying signals to the AKT and MAPK pathways that control glucose uptake, metabolism and growth. Inhibitory serine phosphorylation of IRS1 is a common readout in insulin resistance research.
| Target | Insulin receptor substrate 1 (gene IRS1; UniProt P35568, human) |
|---|---|
| Clone | GIF-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 131.6 kDa |
| Observed MW | 170 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HEK293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human IRS1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places insulin receptor substrate 1 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiuxia Sun et al. (2018) Serum IRS-1 acts as a novel biomarker for diagnosis in patients with nasopharyngeal carcinoma. International Journal of Clinical and Experimental Pathology. PMID: 31949750
