| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin-like protein ISG15; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP; ISG15; G1P2; UCRP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ISG15 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADOH-9 is a rabbit monoclonal antibody that recognizes ubiquitin-like protein ISG15 in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 17.9 kDa, and the supplier reports an observed band at about 15 kDa.
ISG15 is a ubiquitin-like protein strongly induced by type I interferons. It is attached to many target proteins through an E1–E2–E3 enzyme cascade (ISGylation) and can also act in free form, including as a secreted signal. ISG15 is a standard marker of interferon responses and is studied in antiviral immunity.
| Target | Ubiquitin-like protein ISG15 (gene ISG15; UniProt P05161, human) |
|---|---|
| Clone | ADOH-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 17.9 kDa |
| Observed MW | 15 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ISG15.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes ubiquitin-like protein ISG15 as secreted and also located in the cytoplasm. Tissue expression noted by UniProt (human): Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yedan Liao et al. (2023) ISG15 is a potential therapeutic target for osteosarcoma: a comprehensive analysis based on bioinformatics and in vitro experiments. American Journal of Translational Research. PMID: 36915723
