| Field | Specification |
|---|---|
| Target | |
| Alternative names | COP9 signalosome complex subunit 5; SGN5; Signalosome subunit 5; Jun activation domain-binding protein 1; COPS5; CSN5; JAB1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human JAB1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEEG-3 is a rabbit monoclonal antibody that recognizes COP9 signalosome complex subunit 5 (gene COPS5) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 37.6 kDa, and the supplier reports an observed band at about 35 kDa.
COPS5 (CSN5, also called JAB1) is the catalytic subunit of the COP9 signalosome, a metalloprotease that removes NEDD8 from cullins to control cullin-RING ubiquitin ligase activity. It has also been reported to affect the stability of several signaling proteins, and it is studied in protein degradation and cancer biology.
| Target | COP9 signalosome complex subunit 5 (gene COPS5; UniProt Q92905, human) |
|---|---|
| Clone | AEEG-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 37.6 kDa |
| Observed MW | 35 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human MCF-7 cells, human Jurkat cells, human U-87MG cells, human HepG2 cells, rat spleen tissue, rat lung tissue, mouse spleen tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human JAB1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places COP9 signalosome complex subunit 5 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Haomou Pu et al. (2026) Delphinidin, a dietary anthocyanidin, modulates PD-L1 and STING/TBK1 signaling and reprograms the immune microenvironment in triple-negative breast cancer. Journal of Functional Foods. 10.1016/j.jff.2026.107268
