| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Plasminogen activator inhibitor 1;PAI;PAI-1;Endothelial plasminogen activator inhibitor;Serpin E1;SERPINE1;PAI1, PLANH1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human JAG1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-JAG1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 17J22; isotype IgG; reactivity: Human. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-JAG1 Rabbit Monoclonal Antibody catalog # M00640-1. Tested in WB, IHC applications. This antibody reacts with Human.
Key elements and design rationale
- Target: JAG1 (Plasminogen activator inhibitor 1).
- Antibody format: Monoclonal; clone 17J22; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
JAG1 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. . Reported cellular localization context: Secreted. Tissue expression notes (as provided): Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Research relevance and current trends
- Research context keywords from the source record include: Angiogenesis,Cardiovascular.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate JAG1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect JAG1 expression by Western blot in cell or tissue lysates, Detect JAG1 in FFPE tissue sections by immunohistochemistry
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 85 kDa; calculated MW: 45060 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 85 kDa
- Cellular localization (provided): Secreted.
- Tissue details (provided): Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
