| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein kinase JAK1;2.7.10.2;Janus kinase 1;JAK-1;JAK1;JAK1A, JAK1B; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human JAK1, different from the related mouse sequence by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes tyrosine-protein kinase JAK1 in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 133.3 kDa, and the supplier reports an observed band at about 133 kDa.
JAK1 (Janus kinase 1) is a cytoplasmic tyrosine kinase bound to many cytokine receptors, including those for interferons, IL-6 family cytokines and cytokines that use the common gamma chain. Upon cytokine binding, JAK1 phosphorylates the receptor and STAT proteins, which then regulate gene expression, and it is a major target of JAK inhibitor research.
| Target | Tyrosine-protein kinase JAK1 (gene JAK1; UniProt P23458, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 133.3 kDa |
| Observed MW | 133 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat kidney tissue, mouse kidney tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human JAK1. It differs from the mouse sequence at three residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tyrosine-protein kinase JAK1 in the endomembrane system. Tissue expression noted by UniProt (human): Expressed at higher levels in primary colon tumors than in normal colon tissue. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Maoyu Ye et al. (2024) SO2 activates Th17 cells through the JAK1,2/STAT3 signaling pathway. International Immunopharmacology. 10.1016/j.intimp.2024.113263
Honglin Qu et al. (2023) Integration analysis using bioinformatics and experimental validation on the clinical and biological significance of TSLP in cancers. Cellular Signalling. 10.1016/j.cellsig.2023.110874
CHEN Hong-yan et al. (2022) HBP1 inhibits chicken preadipocyte differentiation by activating the STAT3 signaling via directly enhancing JAK2 expression. Journal of Integrative Agriculture. 10.1016/S2095-3119(21)63895-9
Maojun Liu et al. (2018) Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway. International Journal of Molecular Medicine. 10.3892/ijmm.2018.3419
T Ge et al. (2019) MiR-296-3p may affect the proliferation and migration of non-small cell lung cancer cells via regulating RABL3. European Review for Medical and Pharmacological Sciences. 10.26355/eurrev_201907_18321
Xiaodong Song et al. (2012) Changes in cell ultrastructure and inhibition of JAK1/STAT3 signaling pathway in CBRH-7919 cells with astaxanthin. Toxicology Mechanisms and Methods. 10.3109/15376516.2012.717119
