| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2; JAK2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human JAK2 Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone COG-10 is a rabbit monoclonal antibody that recognizes tyrosine-protein kinase JAK2 in human, mouse and rat samples and is validated for immunoprecipitation, ICC/IF, IHC and Western blot. The predicted molecular weight is 130.7 kDa, and the supplier reports an observed band at about 131 kDa.
JAK2 is a cytoplasmic tyrosine kinase of the Janus kinase family that associates with receptors for erythropoietin, thrombopoietin, growth hormone, prolactin, leptin and interferons. Ligand binding activates JAK2, which phosphorylates the receptor and recruits STAT proteins such as STAT5 that then move to the nucleus to switch on target genes. The JAK2 V617F mutation is well known in myeloproliferative neoplasms, so JAK2 is widely studied in hematopoiesis and blood cancers.
| Target | Tyrosine-protein kinase JAK2 (gene JAK2; UniProt O60674, human) |
|---|---|
| Clone | COG-10 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 130.7 kDa |
| Observed MW | 131 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50–1:100 |
Samples with a confirmed band (WB): human HepG2 cells, human THP-1 cells, rat NRK cells, rat PC-12 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): human prostate cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A549 cells.
Immunogen
Synthetic phosphopeptide based on human JAK2 Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tyrosine-protein kinase JAK2 in the endomembrane system, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Ubiquitously expressed throughout most tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yu Yang et al. (2025) Engineered Panax notoginseng polysaccharide micelles inhibit macrophage polarization and delay the progression of rheumatoid arthritis via JAK2-STAT3 signaling pathway. Journal of Nanobiotechnology. 10.1186/s12951-025-03576-8
Bing Xiao et al. (2024) In situ formed reactive oxygen species-responsive dipyridamole prodrug hydrogel: Spatiotemporal drug delivery for chemoimmunotherapy. Journal of Controlled Release. 10.1016/j.jconrel.2024.08.043
Deng Zhicheng et al. (2022) Curcumin and its nano-formulations: Defining triple-negative breast cancer targets through network pharmacology, molecular docking, and experimental verification. Frontiers in Pharmacology. 10.3389/fphar.2022.920514
Chengjie Deng et al. (2024) Sappanone A attenuates rheumatoid arthritis via inhibiting PI3K/AKT/NF-κB and JAK2/STAT3 signaling pathways in vivo and in vitro. International Immunopharmacology. 10.1016/j.intimp.2024.113560
Maoyu Ye et al. (2024) SO2 activates Th17 cells through the JAK1,2/STAT3 signaling pathway. International Immunopharmacology. 10.1016/j.intimp.2024.113263
Bo-Wei Su et al. (2026) FXR-mediated transcriptional regulation of KLF11 mitigates contrast-induced acute kidney injury via suppressing JAK2/STAT3 pathway. International Immunopharmacology. 10.1016/j.intimp.2026.116494
Jiaming He et al. (2020) Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2020.110541
Chang-Peng Xu et al. (2019) ELP2 negatively regulates osteoblastic differentiation impaired by tumor necrosis factor α in MC3T3–E1 cells through STAT3 activation. Journal of Cellular Physiology. 10.1002/jcp.28440
Kaili Liu et al. (2018) Retracted: Hispidulin suppresses cell growth and metastasis by targeting PIM1 through JAK2/STAT3 signaling in colorectal cancer. Cancer Science. 10.1111/cas.13575
Yanfei He et al. (2026) A novel polysaccharide from Polygonatum cyrtonema fibrous root mediates anti-diarrheal effects by reprogramming macrophage M1/M2 polarization. Journal of Functional Foods. 10.1016/j.jff.2026.107250
