| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein kinase JAK3; Janus kinase 3; JAK-3; Leukocyte janus kinase; L-JAK; JAK3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human JAK3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17J71 is a rabbit monoclonal antibody that recognizes tyrosine-protein kinase JAK3 in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 125.1 kDa, and the supplier reports an observed band at about 125 kDa.
JAK3 is a Janus family tyrosine kinase found mainly in blood and immune cells, where it pairs with the common gamma chain shared by receptors for IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21. Working with JAK1, it phosphorylates these receptors and STAT proteins such as STAT5, driving lymphocyte development and activation. Loss of JAK3 causes a form of severe combined immunodeficiency, and it is a target of JAK inhibitor research.
| Target | Tyrosine-protein kinase JAK3 (gene JAK3; UniProt P52333, human) |
|---|---|
| Clone | 17J71 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 125.1 kDa |
| Observed MW | 125 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human JAK3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tyrosine-protein kinase JAK3 in the endomembrane system and cytoplasm. Tissue expression noted by UniProt (human): In NK cells and an NK-like cell line but not in resting T-cells or in other tissues.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
