| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein Jumonji; Jumonji/ARID domain-containing protein 2; JARID2; JMJ |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Jarid2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21J42 is a rabbit monoclonal antibody that recognizes protein Jumonji (gene JARID2) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 138.7 kDa, and the supplier reports an observed band at about 139 kDa.
JARID2 (Jumonji) is an accessory subunit of Polycomb repressive complex 2 (PRC2), which places the repressive H3K27me3 mark on chromatin. Although it carries a Jumonji domain, it lacks demethylase activity; instead it binds DNA and helps recruit PRC2 to target genes in embryonic stem cells. It is essential for embryonic development, including heart, liver, neural tube and blood formation, and is studied in stem cell biology and epigenetics.
| Target | Protein Jumonji (gene JARID2; UniProt Q92833, human) |
|---|---|
| Clone | 21J42 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 138.7 kDa |
| Observed MW | 139 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): NCCIT cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human jarid2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places protein Jumonji in the nucleus. Tissue expression noted by UniProt (human): During embryogenesis, predominantly expressed in neurons and particularly in dorsal root ganglion cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
