| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase 8;MAP kinase 8;MAPK 8;2.7.11.24;JNK-46;Stress-activated protein kinase 1c;SAPK1c;Stress-activated protein kinase JNK1;c-Jun N-terminal kinase 1;MAPK8;JNK1, PRKM8, SAPK1, SAPK1C; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human JNK1+JNK2+JNK3 |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DFI-13 is a rabbit monoclonal antibody that recognizes MAPK8 in human, mouse and rat samples and is validated for Western blot, ICC/IF and immunoprecipitation.
| Target | MAPK8 (UniProt P45983/P45984/P53779) |
|---|---|
| Clone | DFI-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Observed MW | 40, 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human U87 cells, human A549 cells, rat PC-12 cells, rat C6 cells, mouse Neuro-2a cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human JNK1+JNK2+JNK3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chuandong Cai et al. (2025) Zwitterionic Brush-Grafted Interfacial Bio-Lubricant Evades Complement C3-Mediated Macrophage Phagocytosis for Osteoarthritis Therapy. Advanced Materials. 10.1002/adma.202501137
Shangguan Yangfan et al. (2017) Glucocorticoid mediates prenatal caffeine exposure-induced endochondral ossification retardation and its molecular mechanism in female fetal rats. Cell Death & Disease. 10.1038/cddis.2017.546
Hongjing Zhao et al. (2019) The cardiotoxicity of the common carp (Cyprinus carpio) exposed to environmentally relevant concentrations of arsenic and subsequently relieved by zinc supplementation. Environmental Pollution. 10.1016/j.envpol.2019.07.065
Song Wang et al. (2023) Identification of several inflammation-related genes based on bioinformatics and experiments. International Immunopharmacology. 10.1016/j.intimp.2023.110409
Gui-zhou Zheng et al. (2022) Dioscin induces osteosarcoma cell apoptosis by upregulating ROS-mediated P38 MAPK signaling. Drug Development Research. 10.1002/ddr.22009
Wei Zhang et al. (2019) Lower range of molecular weight of xanthan gum inhibits apoptosis of chondrocytes through MAPK signaling pathways. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2019.01.071
Ye Ding et al. (2011) Study of Histopathological and Molecular Changes of Rat Kidney under Simulated Weightlessness and Resistance Training Protective Effect. PLoS One. 10.1371/journal.pone.0020008
Zhou Can-Can et al. (2022) Zinc supplementation ameliorates sorafenib-induced cognitive impairment through ROS/JNK signaling pathway. Biological Trace Element Research. 10.1007/s12011-022-03142-5
Xuefeng Bu et al. (2016) Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress. American Journal of Cancer Research. PMID: 27293989
Xianglong Meng et al. (2024) Efficacy of Rhamnus utilis Decne. aqueous extract in mice with acute alcoholic liver injury and metabolomic study. Heliyon. 10.1016/j.heliyon.2024.e32523
