| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase 8;MAP kinase 8;MAPK 8;2.7.11.24;JNK-46;Stress-activated protein kinase 1c;SAPK1c;Stress-activated protein kinase JNK1;c-Jun N-terminal kinase 1;MAPK8;JNK1, PRKM8, SAPK1, SAPK1C; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human JNK1, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes mitogen-activated protein kinase 8 (gene MAPK8) in rat, mouse and human samples and is validated for Western blot. The predicted molecular weight is 48.3 kDa, and the supplier reports an observed band at about 60 kDa.
JNK1 (MAPK8) is a stress-activated serine/threonine kinase of the c-Jun N-terminal kinase family. Cytokines, UV light and other stresses switch it on through the upstream kinases MKK4 and MKK7, after which it phosphorylates c-Jun, ATF2 and other AP-1 components. It is widely studied in apoptosis, inflammation, T-helper cell differentiation and metabolic stress.
| Target | Mitogen-activated protein kinase 8 (gene MAPK8; UniProt P45983, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat, Mouse, Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 48.3 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): HT1080 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the N-terminal region of human JNK1. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with rat, mouse and human. UniProt places mitogen-activated protein kinase 8 in the cytoplasm, nucleus and synapse. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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