| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase 10;MAP kinase 10;MAPK 10;2.7.11.24;MAP kinase p49 3F12;Stress-activated protein kinase 1b;SAPK1b;Stress-activated protein kinase JNK3;c-Jun N-terminal kinase 3;MAPK10;JNK3, JNK3A, PRKM10, SAPK1B; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human JNK3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HGC-13 is a rabbit monoclonal antibody that recognizes mitogen-activated protein kinase 10 (gene MAPK10) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 52.6 kDa; the supplier reports observed bands at 50,53 kDa.
JNK3 (MAPK10) is a c-Jun N-terminal kinase expressed mainly in the brain, heart and testis. Activated by stress and inflammatory cytokines through MKK4 and MKK7, it phosphorylates c-Jun and other targets, and it is studied for its role in stress-induced neuronal death and neurodegeneration.
| Target | Mitogen-activated protein kinase 10 (gene MAPK10; UniProt P53779, human) |
|---|---|
| Clone | HGC-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 52.6 kDa |
| Observed MW | 50,53 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human JNK3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitogen-activated protein kinase 10 in the cytoplasm, membrane, nucleus and mitochondrion. Tissue expression noted by UniProt (human): Specific to a subset of neurons in the nervous system. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Guangyi et al. (2013) Astragalus injection protects cerebral ischemic injury by inhibiting neuronal apoptosis and the expression of JNK3 after cerebral ischemia reperfusion in rats. Behavioral and Brain Functions. 10.1186/1744-9081-9-36
