| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Thy-1 membrane glycoprotein; CDw90; Thy-1 antigen; CD90; THY1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human JUNB, which shares 88.9% amino acid (aa) sequence identity with both mouse and rat JUNB. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-JUNB Antibody Picoband® is an antibody for JUNB detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: JUNB (Thy-1 cell surface antigen); UniProt: P17275
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 42-45 kDa, calculated 18080 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-JUNB Antibody Picoband® catalog # A01825-3.
Biological background
Biological context: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Expression and localization notes: cellular localization: Cell membrane; Lipid-anchor, GPI-anchor., tissue context: Detected adjacent to nodes of Ranvier in juxtaparanodal zones in spinal cord nerve fibers, but also in paranodal regions in some myelinated spinal cord axons (at protein level) (PubMed:11086297). Detected in the islet of Langerhans (PubMed:21483673)..
Common research applications
- Western blotting (WB): Compare JUNB levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of JUNB in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify JUNB-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Transcription factor jun-B (JUNB) is a protein that in humans is encoded by the JUNB gene. It is mapped to 19p13.2. JUNB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3', and a large fraction (over 50%) of the JUNB locus is contained in these flanking evolutionarily conserved sequences (FECS), which may be required for effecting the proper transcriptional regulation of this gene. What's more, the expression of JUNB gene might be involved in terminal granulocyte differentiation or in regulating granulocyte functionality.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane; Lipid-anchor, GPI-anchor.
- Tissue details: Detected adjacent to nodes of Ranvier in juxtaparanodal zones in spinal cord nerve fibers, but also in paranodal regions in some myelinated spinal cord axons (at protein level) (PubMed:11086297). Detected in the islet of Langerhans (PubMed:21483673).
- Research category: Neuroscience,Stem Cells,Synapse Marker,T Cells,T Lymphocytic Lineage
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
