| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor jun-D;JUND; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human JunD |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HHI-10 is a rabbit monoclonal antibody that recognizes transcription factor JunD in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 35.2 kDa; the supplier reports observed bands at 38, 42 kDa.
JunD (JUND) is a member of the Jun family of bZIP transcription factors. It dimerizes with Fos family proteins to form AP-1 complexes that bind TGA[GC]TCA elements and regulate gene expression. JunD is broadly expressed and is studied in stress and oxidative responses, cell proliferation and survival.
| Target | Transcription factor JunD (gene JUND; UniProt P17535, human) |
|---|---|
| Clone | HHI-10 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.2 kDa |
| Observed MW | 38, 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human junD.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor JunD in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
