| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcriptional regulator Kaiso; Zinc finger and BTB domain-containing protein 33; ZBTB33; KAISO; ZNF348 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KAISO |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23Z48 is a rabbit monoclonal antibody that recognizes transcriptional regulator Kaiso (gene ZBTB33) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 74.5 kDa, and the supplier reports an observed band at about 85 kDa.
Kaiso (ZBTB33) is a BTB/POZ zinc finger transcription factor that binds both methylated CpG sequences and a non-methylated Kaiso binding site. It recruits the N-CoR corepressor complex to repress target genes and interacts with p120 catenin, linking cell adhesion signaling to gene regulation, and it is studied in epigenetics and Wnt signaling.
| Target | Transcriptional regulator Kaiso (gene ZBTB33; UniProt Q86T24, human) |
|---|---|
| Clone | 23Z48 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 74.5 kDa |
| Observed MW | 85 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human KAISO.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcriptional regulator Kaiso in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in vascular endothelium. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
