| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription intermediary factor 1-beta;TIF1-beta;E3 SUMO-protein ligase TRIM28;6.3.2.-;KRAB-associated protein 1;KAP-1;KRAB-interacting protein 1;KRIP-1;Nuclear corepressor KAP-1;RING finger protein 96;Tripartite motif-containing protein 28;TRIM28;KAP1, RNF96, TIF1B; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transcription intermediary factor 1-beta (gene TRIM28) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 88.6 kDa, and the supplier reports an observed band at about 100 kDa.
TRIM28 (KAP1, TIF1β) is a transcriptional corepressor and E3 SUMO/ubiquitin ligase that is recruited to DNA by KRAB zinc finger proteins. It assembles repressive complexes containing SETDB1, HP1 and histone deacetylases to silence endogenous retroviruses and other repeats and to protect genomic imprinting, and it also takes part in the DNA damage response.
| Target | Transcription intermediary factor 1-beta (gene TRIM28; UniProt Q13263, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 88.6 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human PC-3 cells, human 293T cells, human A549 cells, rat PC-12 cells, mouse spleen tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue, human mammary cancer tissue, human colorectal adenocarcinoma tissue, human gastric carcinoma tissue, human liver cancer tissue, human ovarian cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human ovarian cancer tissue.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human KAP1 fragment (Ala699–Pro835), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 94.9%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription intermediary factor 1-beta in the nucleus. Tissue expression noted by UniProt (human): Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
