| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Histone acetyltransferase KAT2A;2.3.1.48;General control of amino acid synthesis protein 5-like 2;Histone acetyltransferase GCN5;HsGCN5;Lysine acetyltransferase 2A;STAF97;KAT2A;GCN5, GCN5L2, HGCN5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human KAT2A/GCN5, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of KAT2A (Histone acetyltransferase KAT2A) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-KAT2A/GCN5 Antibody Picoband® catalog # PB9713. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human KAT2A/GCN5, identical to the related mouse and rat sequences.
- Molecular weight context: reported MW: 94 kDa; calculated MW: 93926 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Histone acetyltransferase KAT2A; Histone acetyltransferase KAT2A. Histone acetyltransferase KAT2A?is an?enzyme?that in humans is encoded by the?KAT2A?gene. It is mapped to 17q21. KAT2A, or GCN5, GCN5L2, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. The GCN5 protein, a regulator of transcription activation in yeast, promotes maximal levels of transcription by 2 transcriptional activators, GCN4 and the HAP2-HAP3-HAP4 complex.?The GCN4 protein activates transcription of a large number of amino acid biosynthetic genes under limiting amino acid conditions and the HAP2-HAP3-HAP4 complex is thought to mediate transcription of genes involved in respiratory functions.?GCN5 also functions as a repressor of NF-kappa-B by promoting ubiquitination of the NF-kappa-B subunit RELA in a HAT-independent manner. Functional note: Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. Also acetylates non- histone proteins, such as CEBPB (PubMed:17301242). Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. . Reported localization: Nucleus . Expression/tissue context: Expressed in all tissues tested, with most abundant expression in ovary.
Research relevance and current trends
- Acetylation: Researchers commonly examine how KAT2A (Histone acetyltransferase KAT2A) relates to this theme using model systems and orthogonal readouts.
- Chromatin Modifying Enzymes: Researchers commonly examine how KAT2A (Histone acetyltransferase KAT2A) relates to this theme using model systems and orthogonal readouts.
- Co-Factors: Researchers commonly examine how KAT2A (Histone acetyltransferase KAT2A) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative KAT2A (Histone acetyltransferase KAT2A) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
