| Field | Specification |
|---|---|
| Target | |
| Gene symbol | |
| Gene ID | |
| Accession number | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Internal |
| Conjugate | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This polyclonal antibody, raised in Goat, recognizes KCNB1. It is validated for Pep-ELISA.
Validated Applications
| Applications | Pep-ELISA |
|---|---|
| Peptide ELISA | antibody detection limit dilution 1:4000. |
| WB | Preliminary experiments gave a 100kDa band and additional stronger bands at approx 50kDa and 38kDa in Human Brain (Cerebellum) and Heart lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the extra bands we observe given the calculated size of 95.9kDa according to NP_004966.1. Both detected extra bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field-have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
See specifications table.
Specifications
| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Dog, Cow, Pig |
| Immunogen Region | Internal |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration | 0.5 mg/mL |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA NA |
| Gene ID | 3745 |
| Storage | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Human, Mouse, Dog, Cow, Pig. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for KCNB1 research from St John's Laboratory Ltd, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
