| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Potassium channel subunit β MiRP2, MinK-related peptide 2, Potassium voltage-gated channel subfamily E member 3, HOKPP |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-KCNE3 (MiRP2) Antibody is an antibody targeting Potassium channel subunit β MiRP2, MinK-related peptide 2, Potassium voltage-gated channel subfamily E member 3, HOKPP Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IC, IF, IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Potassium channel subunit β MiRP2, MinK-related peptide 2, Potassium voltage-gated channel subfamily E member 3, HOKPP (also reported as Potassium channel subunit β MiRP2, MinK-related peptide 2, Potassium voltage-gated channel subfamily E member 3, HOKPP).
- Immunogen/epitope region: Intracellular, C-terminal domain.
- Homology note: Rat, mouse, dog, pig - identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- KO-validated: yes (validation context may be assay-dependent).
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
KCNE3 (or MiRP2) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. Other members of the family are KCNE1 (IsK, MiNK), KCNE2 (MiRP1), KCNE4 (MiRP3) and KCNE5 (MiRP4).KCNE1 is the founding member of the family and was initially believed to form a K+ channel itself, but was later recognized to work as a regulatory b subunit associated with the KV7.1 (KCNQ1) a protein. KCNE3 was discovered based on its homology with KCNE1.The KCNE regulatory subunits are small proteins (14-20 kD) with a type-1 integral membrane topology.
Research relevance and current trends
- Linking transporter/channel abundance to ionic homeostasis and excitability-related phenotypes.
- Studying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.
- Combining antibody readouts with functional assays for more complete interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Conceptual control: KO/KD samples provide orthogonal support for target assignment when available.
- Provided control suggestions: Negative control: BLP-PC118.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-PC118; Negative control: BLP-PC118.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
