Anti-KCTD9 Rabbit Monoclonal Antibody

SKU:BHA21010472
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Boster Bio
Boster Bio
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Overview
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Anti-KCTD9 antibody (Rabbit monoclonal, clone 29K82, IgG). Validated for Western blot and immunofluorescence. Reactivity: Human. Recognizes BTB/POZ domain-containing protein KCTD9. Observed band in Western blot: about 43 kDa.
Target KCTD9
clone number 29K82
Host Rabbit
Reactivity Human
Isotype IgG
Application(s) WB, IF, ICC
Options selector
Catalog no. Size Conjugation
M12515-1 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      • Form: Liquid
      • Applications: WB,ICC,IF
      • Application details: WB 1:500-2000
        ICC/IF 1:50-200
      • Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target KCTD9
Alternative names BTB/POZ domain-containing protein KCTD9; KCTD9
Gene ID 54793
UniProt # Q7L273
Host Rabbit
Clone 29K82
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Immunofluorescence
Immunogen A synthesized peptide derived from human KCTD9
Molecular weight 42.6 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus. Chromosome.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M12515-1
Main SKU BHA21010472
Rabbit Monoclonal

Product Overview

Clone 29K82 is a rabbit monoclonal antibody that recognizes BTB/POZ domain-containing protein KCTD9 in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 42.6 kDa, and the supplier reports an observed band at about 43 kDa.

KCTD9 is a BTB/POZ domain protein of the KCTD family that acts as a substrate adapter for a CUL3-based E3 ubiquitin ligase, directing target proteins to ubiquitination and proteasomal degradation. Its specific substrates and physiological roles are still being characterized.

Target BTB/POZ domain-containing protein KCTD9 (gene KCTD9; UniProt Q7L273, human)
Clone 29K82
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 42.6 kDa
Observed MW 43 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunocytochemistry / Immunofluorescence 1:50–1:200

Samples with a confirmed band (WB): HepG2 cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human KCTD9.

Reactivity Notes

The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunocytochemistry / Immunofluorescence 1:50–1:200. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 42.6 kDa; observed band at about 43 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human KCTD9.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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