| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lysine-specific demethylase 4A; JmjC domain-containing histone demethylation protein 3A; Jumonji domain-containing protein 2A; [histone H3]-trimethyl-L-lysine(36) demethylase 4A; [histone H3]-trimethyl-L-lysine(9) demethylase 4A; KDM4A; JHDM3A; JMJD2; JMJD2A; KIAA0677 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KDM4A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEHB-11 is a rabbit monoclonal antibody that recognizes lysine-specific demethylase 4A (gene KDM4A) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 120.7 kDa, and the supplier reports an observed band at about 150 kDa.
KDM4A (JMJD2A) is a JmjC domain histone demethylase that uses Fe(II) and 2-oxoglutarate to remove trimethyl marks from histone H3 at Lys9 and Lys36. By altering these marks it affects heterochromatin, transcription and DNA replication timing, and it has been linked to genome instability. KDM4A is studied in epigenetics and as a potential cancer target.
| Target | Lysine-specific demethylase 4A (gene KDM4A; UniProt O75164, human) |
|---|---|
| Clone | AEHB-11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 120.7 kDa |
| Observed MW | 150 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:60 |
Immunogen
Synthetic peptide based on the sequence of human KDM4A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places lysine-specific demethylase 4A in the nucleus. Tissue expression noted by UniProt (human): Ubiquitous.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
