Anti-KDM4B / JMJD2B Rabbit Monoclonal Antibody

Overview

This product is an anti-KDM4B antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23K44; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-KDM4B / JMJD2B Rabbit Monoclonal Antibody catalog # M04127. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: KDM4B (MICOS complex subunit MIC60).
• Antibody format: Monoclonal; clone 23K44; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

KDM4B (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:22114354, PubMed:25781180). . Reported cellular localization context: Mitochondrion inner membrane ; Single-pass membrane protein . Mitochondrion . Tissue expression notes (as provided): Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells. .

Research relevance and current trends

• Research context keywords from the source record include: Cancer,Cardiovascular,Metabolism,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Pathways and Processes,Signal Transduction.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.

Workflow ideas (metafield): Validate KDM4B antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect KDM4B expression by Western blot in cell or tissue lysates, Detect KDM4B in FFPE tissue sections by immunohistochemistry, Localize KDM4B by immunofluorescence/immunocytochemistry in cultured cells, Quantify KDM4B-positive cells by flow cytometry in single-cell suspensions

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 65 kDa, 75 kDa; calculated MW: 83678 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 65 kDa, 75 kDa
• Cellular localization (provided): Mitochondrion inner membrane ; Single-pass membrane protein . Mitochondrion .
• Tissue details (provided): Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.