| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lysine-specific demethylase 6B; JmjC domain-containing protein 3; Jumonji domain-containing protein 3; Lysine demethylase 6B; KDM6B; JMJD3; KIAA0346 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human KDM6B/JMJD3 recombinant protein (Position: R1127-R1643). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets lysine-specific demethylase 6B (gene KDM6B) in human and mouse samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 176.6 kDa, and the supplier reports an observed band at about 177 kDa.
JMJD3 (KDM6B) is a JmjC domain histone demethylase that removes di- and trimethyl marks from histone H3 at Lys27, relieving Polycomb-mediated gene silencing. It is rapidly induced by inflammatory signals and helps activate genes in macrophages, and it also regulates HOX genes and cell differentiation during development. It is studied in inflammation, immunity and cancer epigenetics.
| Target | Lysine-specific demethylase 6B (gene KDM6B; UniProt O15054, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 176.6 kDa |
| Observed MW | 177 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human A375 cells, human HEK293 cells, human A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): K562 cells, HEPA1–6 cells.
Immunogen
Recombinant human KDM6B/JMJD3 fragment (Arg1127–Arg1643), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places lysine-specific demethylase 6B in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Jin et al. (2024) JMJD3 regulate H3K27me3 modification via interacting directly with TET1 to affect spermatogonia self-renewal and proliferation. BMC Genomics. 10.1186/s12864-024-10120-9
