| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bifunctional peptidase and arginyl-hydroxylase JMJD5; JmjC domain-containing protein 5; Jumonji C domain-containing protein 5; L-arginine (3R)-hydroxylase KDM8; KDM8; JMJD5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human KDM8 recombinant protein (Position: D4-D406). Human KDM8 shares 78.1% and 78.9% amino acid (aa) sequence identity with mouse and rat KDM8, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets bifunctional peptidase and arginyl-hydroxylase JMJD5 (gene KDM8) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 47.3 kDa, and the supplier reports an observed band at about 55 kDa.
JMJD5 (KDM8) is a JmjC domain enzyme that uses 2-oxoglutarate and Fe(II). It has been described as an arginyl hydroxylase and as a protease that clips histone tails at methylated arginine or lysine residues, which may help release paused RNA polymerase II. JMJD5 is required for embryonic development and has been linked to cell cycle progression and circadian rhythm.
| Target | Bifunctional peptidase and arginyl-hydroxylase JMJD5 (gene KDM8; UniProt Q8N371, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 47.3 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human KDM8 fragment (Asp4–Asp406), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 78.1% and 78.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places bifunctional peptidase and arginyl-hydroxylase JMJD5 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Weakly expressed in most cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
