| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KGF |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26F04 is a rabbit monoclonal antibody that recognizes fibroblast growth factor 7 (gene FGF7) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 22.5 kDa, and the supplier reports an observed band at about 28 kDa.
Fibroblast growth factor 7 (FGF7), also called keratinocyte growth factor (KGF), is produced by mesenchymal cells and acts on epithelial cells through FGFR2b. It stimulates the proliferation and repair of keratinocytes and other epithelia, and it is studied in wound healing, epithelial protection and branching morphogenesis.
| Target | Fibroblast growth factor 7 (gene FGF7; UniProt P21781, human) |
|---|---|
| Clone | 26F04 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.5 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human 293T cells, human A549 cells, human HT1080 cells, rat lung tissue, rat NRK cells, mouse lung tissue, mouse NIH/3T3 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human KGF.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes fibroblast growth factor 7 as a secreted protein. Tissue expression noted by UniProt (human): Epithelial cell. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Xinping et al. (2013) The effects of adenoviral transfection of the keratinocyte growth factor gene on epidermal stem cells: An in vitro study. Molecules and Cells. 10.1007/s10059-013-0093-y
