| Field | Specification |
|---|---|
| Target | |
| Alternative names | Far upstream element-binding protein 2; FUSE-binding protein 2; KH type-splicing regulatory protein; KSRP; p75; KHSRP; FUBP2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human KHSRP recombinant protein (Position: D116-R478). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects far upstream element-binding protein 2 (gene KHSRP) in human and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry, ELISA and immunoprecipitation. The predicted molecular weight is 73.1 kDa, and the supplier reports an observed band at about 85–90 kDa.
KHSRP (also called FUBP2 or KSRP) is a single-stranded nucleic acid-binding protein with four KH domains. It promotes decay of mRNAs that contain AU-rich elements, helps process certain microRNA precursors, and influences alternative splicing, so it has a broad role in post-transcriptional control of gene expression, including genes involved in inflammation.
| Target | Far upstream element-binding protein 2 (gene KHSRP; UniProt Q92945, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 73.1 kDa |
| Observed MW | 85–90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEK293 cells, human HeLa cells, human MCF-7 cells, human Jurkat cells, human K562 cells, human Caco-2 cells, human U-937 cells, rat brain tissue, rat stomach tissue, mouse brain tissue, mouse stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human gallbladder adenocarcinoma tissue, human laryngeal squamous cells carcinoma tissue, human liver cancer tissue, human lung cancer tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): Caco-2 cells.
Samples with a confirmed result (IP): K562 cells.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Recombinant human KHSRP fragment (Asp116–Arg478), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places far upstream element-binding protein 2 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Detected in neural and non-neural cell lines. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
