| Field | Specification |
|---|---|
| Target | |
| Alternative names | Antigen KI-67;MKI67; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Ki67 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EAH-13 is a rabbit monoclonal antibody that recognizes proliferation marker protein Ki-67 (gene MKI67) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 358.7 kDa, and the supplier reports an observed band at about 359 kDa.
Ki-67 (MKI67) is a large nuclear protein present in dividing cells but absent in resting (G0) cells. During mitosis it coats the chromosome surface and helps keep individual chromosomes separated, and because its expression tracks cell division, Ki-67 is one of the most widely used markers of cell proliferation.
| Target | Proliferation marker protein Ki-67 (gene MKI67; UniProt P46013, human) |
|---|---|
| Clone | EAH-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 358.7 kDa |
| Observed MW | 359 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): Ramos cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human pituitary tumor tissue, human liver cancer tissue, human tonsil tissue, human esophageal carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human ki67.
Reactivity Notes
The supplier lists reactivity with human. UniProt places proliferation marker protein Ki-67 in the chromosome and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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