| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interferon regulatory factor 9;IRF-9;IFN-alpha-responsive transcription factor subunit;ISGF3 p48 subunit;Interferon-stimulated gene factor 3 gamma;ISGF-3 gamma;Transcriptional regulator ISGF3 subunit gamma;IRF9;ISGF3G; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived human KIF4A/4B recombinant protein (Position: K729-D1174). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-KIF4A/4B Antibody Picoband® is an antibody for KIF4A/4B detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: KIF4A/4B (Interferon regulatory factor 9); UniProt: O95239/Q2VIQ3; NCBI Gene: 24137/285643
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 140 kDa, calculated 43696 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-KIF4A/4B Antibody Picoband® catalog # A04486-1.
Biological background
Biological context: Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Expression and localization notes: cellular localization: Cytoplasm . Nucleus . Translocated into the nucleus upon activation by IFN-alpha/beta., tissue context: Leukocytes..
Common research applications
- Western blotting (WB): Compare KIF4A/4B levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of KIF4A/4B in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify KIF4A/4B-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Chromosome-associated kinesin KIF4A is a protein that in humans is encoded by the KIF4A gene. This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm . Nucleus . Translocated into the nucleus upon activation by IFN-alpha/beta.
- Tissue details: Leukocytes.
- Research category: Cell Adhesion,Cell Type Markers,Cytoskeleton/ECM,Hematopoietic Progenitors,Immunology,Integrins,Myeloid,Signal Transduction,Stem Cells
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
