| Field | Specification |
|---|---|
| Target | |
| Alternative names | Kinesin-1 heavy chain; Conventional kinesin heavy chain; Ubiquitous kinesin heavy chain; UKHC; KIF5B; KNS; KNS1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KIF5B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29K30 is a rabbit monoclonal antibody that recognizes kinesin-1 heavy chain (gene KIF5B) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 109.7 kDa, and the supplier reports an observed band at about 120 kDa.
KIF5B is the ubiquitously expressed heavy chain of kinesin-1, the conventional motor that carries cargo toward microtubule plus ends. It distributes mitochondria, lysosomes and other organelles through the cytoplasm, and it also helps position the nucleus and centrosomes before mitosis. KIF5B is a well-known fusion partner of RET and ALK in lung cancer.
| Target | Kinesin-1 heavy chain (gene KIF5B; UniProt P33176, human) |
|---|---|
| Clone | 29K30 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 109.7 kDa |
| Observed MW | 120 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human KIF5B.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places kinesin-1 heavy chain in the cytoplasm, cytolytic granule membrane and lysosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
