| Field | Specification |
|---|---|
| Target | |
| Alternative names | Kinesin-like protein KIF7; KIF7 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human KIF7, identical to the related mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes kinesin-like protein KIF7 in human samples and is validated for Western blot. The predicted molecular weight is 150.6 kDa, and the supplier reports an observed band at about 151 kDa.
KIF7 is a kinesin-4 family protein that concentrates at the tip of the primary cilium and acts as both a positive and negative regulator of Hedgehog signaling. It organizes the ciliary tip and controls the localization of SUFU–GLI complexes, thereby shaping GLI-dependent gene activity. KIF7 mutations cause ciliopathies such as Joubert and acrocallosal syndromes.
| Target | Kinesin-like protein KIF7 (gene KIF7; UniProt Q2M1P5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 150.6 kDa |
| Observed MW | 151 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human 293T cells, human A375 cells, human HeLa cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human KIF7. The peptide sequence is conserved in mouse.
Reactivity Notes
The supplier lists reactivity with human. UniProt places kinesin-like protein KIF7 in the cell projection. Tissue expression noted by UniProt (human): Embryonic stem cells, melanotic melanoma and Jurkat T-cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
