| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA/RNA-binding protein KIN17;Binding to curved DNA;KIN, antigenic determinant of recA protein homolog;KIN ;BTCD, KIN17; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human KIN recombinant protein (Position: K3-K202). Human KIN shares 98% amino acid (aa) sequence identity with mouse KIN. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes DNA/RNA-binding protein KIN17 in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 45.4 kDa, and the supplier reports an observed band at about 45 kDa.
KIN17 (KIN) is a conserved nuclear protein that binds DNA and RNA. It associates with DNA replication sites and is involved in the cellular response to DNA damage, and it has also been linked to RNA processing and splicing. KIN17 is studied in DNA replication, genome stability and cell proliferation.
| Target | DNA/RNA-binding protein KIN17 (gene KIN; UniProt O60870, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 45.4 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human 293T cells, human HepG2 cells, rat RH35 cells, rat NRK cells, mouse HEPA1–6 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human oesophagus squama cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed result (IP): MCF-7 cells.
Immunogen
Recombinant human KIN fragment (Lys3–Lys202), expressed in E. coli. Sequence identity with the mouse ortholog: 98%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA/RNA-binding protein KIN17 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Junyu Chen et al. (2021) Analysis of the association between KIN17 expression and the clinical features/prognosis of epithelial ovarian cancer, and the effects of KIN17 in SKOV3 cells. Oncology Letters. 10.3892/ol.2021.12736
