| Field | Specification |
|---|---|
| Target | |
| Alternative names | Inward rectifier potassium channel 2; Cardiac inward rectifier potassium channel; Inward rectifier K(+) channel Kir2.1; IRK-1; hIRK1; Potassium channel, inwardly rectifying subfamily J member 2; KCNJ2; IRK1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Kir2.1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25K45 is a rabbit monoclonal antibody that recognizes inward rectifier potassium channel 2 (gene KCNJ2) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 48.3 kDa, and the supplier reports an observed band at about 53 kDa.
Kir2.1 (KCNJ2) is a strong inward rectifier potassium channel that passes potassium into cells more easily than out, because internal Mg2+ and polyamines block outward flow. It helps set the resting membrane potential and shape late repolarization in heart and skeletal muscle and in neurons. Mutations in KCNJ2 cause Andersen–Tawil syndrome.
| Target | Inward rectifier potassium channel 2 (gene KCNJ2; UniProt P63252, human) |
|---|---|
| Clone | 25K45 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 48.3 kDa |
| Observed MW | 53 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human kir2.1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places inward rectifier potassium channel 2 in the cell membrane. Tissue expression noted by UniProt (human): Heart, brain, placenta, lung, skeletal muscle, and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Peng Zhang et al. (2018) Effects of Pinocembrin Pretreatment on Connexin 43 (Cx43) Protein Expression After Rat Myocardial Ischemia-Reperfusion and Cardiac Arrhythmia. Medical Science Monitor. 10.12659/MSM.909162
Juan Huang et al. (2024) Gastrodin Regulates Cardiac Arrhythmia by Targeting the Gap Junction Alpha-1 Protein after Ischemia-Reperfusion. Tohoku Journal of Experimental Medicine. 10.1620/tjem.2024.J030
Guang-ming Zhang et al. (2018) Effect of pinocembrin pre-treatment on expressions of Cx43 protein and claudin 1 in myocardial ischemia cardiomyocytes of arrhythmic rats. Tropical Journal of Pharmaceutical Research. 10.4314/tjpr.v17i3.5
