Anti-KLC1 Rabbit Monoclonal Antibody

Overview

This product is an anti-KLC1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 25K41; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-KLC1 Rabbit Monoclonal Antibody catalog # M04116. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: KLC1 (MICOS complex subunit MIC60).
• Antibody format: Monoclonal; clone 25K41; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

KLC1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:22114354, PubMed:25781180). . Reported cellular localization context: Mitochondrion inner membrane ; Single-pass membrane protein . Mitochondrion . Tissue expression notes (as provided): Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells. .

Research relevance and current trends

• Research context keywords from the source record include: Cancer,Cardiovascular,Metabolism,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Pathways and Processes,Signal Transduction.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate KLC1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect KLC1 expression by Western blot in cell or tissue lysates, Detect KLC1 in FFPE tissue sections by immunohistochemistry, Localize KLC1 by immunofluorescence/immunocytochemistry in cultured cells, Quantify KLC1-positive cells by flow cytometry in single-cell suspensions, Enrich KLC1 by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 65 kDa; calculated MW: 65 kDa).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 65 kDa
• Cellular localization (provided): Mitochondrion inner membrane ; Single-pass membrane protein . Mitochondrion .
• Tissue details (provided): Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.