| Field | Specification |
|---|---|
| Target | |
| Alternative names | Kinesin light chain 1; KLC 1; KLC1; KLC; KNS2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KLC1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25K41 is a rabbit monoclonal antibody that recognizes kinesin light chain 1 (gene KLC1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 65.3 kDa, and the supplier reports an observed band at about 65 kDa.
Kinesin light chain 1 (KLC1) is a subunit of kinesin-1, the conventional microtubule plus-end motor. Two light chains bind the heavy chain tails and, through their tetratricopeptide repeats, connect the motor to cargo adapters and help regulate motor activity. KLC1 is studied in axonal transport and neurodegeneration.
| Target | Kinesin light chain 1 (gene KLC1; UniProt Q07866, human) |
|---|---|
| Clone | 25K41 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 65.3 kDa |
| Observed MW | 65 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human PC-3 cells, human RT4 cells, human MCF-7 cells, human SIHA cells, rat brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human KLC1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places kinesin light chain 1 in the cell projection, cytoplasmic vesicle and cytoplasm. Tissue expression noted by UniProt (human): Found in a variety of tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
