| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor;KLF4;EZF, GKLF; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human KLF4 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-KLF4/Gklf Rabbit Monoclonal Antibody is an antibody targeting KLF4. Common applications include WB, IHC, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AODA-11; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 70 kDa; calculated MW: 54671 MW.
Boster Bio Anti-KLF4/Gklf Rabbit Monoclonal Antibody catalog # M00120. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: KLF4 — Krueppel-like factor 4
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AODA-11; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 70 kDa; Calculated: 54671 MW
Biological background
Protein function (datasheet): Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. .
Cellular localization (datasheet): Nucleus.
Tissue details (datasheet): Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cardiovascular,Cell Cycle,Cell Cycle Inhibitors,Cell Differentiation,Developmental Biology,Domain Families,Embryogenesis,Embryonic Stem Cells,Epigenetics and Nuclear Signaling,Hypertrophy,Intracellular,Krueppel Like Factor,Oncoproteins,Oncoproteins/Suppressors,Stem Cells,Surface Molecules,Transcription,Transcription Factors,Tumor Suppressors.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
