| Field | Specification |
|---|---|
| Target | |
| Alternative names | Klotho; KL |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Klotho |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18K09 is a rabbit monoclonal antibody that recognizes klotho in human and mouse samples and is validated for Western blot and IHC. The predicted molecular weight is 116.2 kDa, and the supplier reports an observed band at about 62 kDa.
Klotho (KL) is a single-pass membrane protein expressed mainly in the kidney that also exists in a soluble form. It acts as a co-receptor that allows FGF23 to signal through FGF receptors, thereby regulating phosphate excretion and vitamin D metabolism. Mice lacking Klotho show features resembling premature aging, making it a subject of aging, kidney and mineral metabolism research.
| Target | Klotho (gene KL; UniProt Q9UEF7, human) |
|---|---|
| Clone | 18K09 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 116.2 kDa |
| Observed MW | 62 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:1000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human fetal kidney.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human klotho.
Reactivity Notes
The supplier lists reactivity with human and mouse. Tissue expression noted by UniProt (human): Present in cortical renal tubules (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
