| Field | Specification |
|---|---|
| Target | |
| Alternative names | Natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; CD antigen CD94; Klrd1; Cd94 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Klrd1/CD94 recombinant protein (Position: N33-I179). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects natural killer cells antigen CD94 (gene Klrd1) in mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 20.8 kDa, and the supplier reports an observed band at about 30 kDa.
CD94 (KLRD1) is a C-type lectin-like receptor subunit on natural killer cells and some cytotoxic T cells. It pairs with NKG2A to form an inhibitory receptor or with NKG2C to form an activating receptor, and both recognize HLA-E loaded with peptides from other MHC class I leader sequences. This allows lymphocytes to check whether cells express normal MHC class I, and CD94 is widely used as an NK cell marker.
| Target | Natural killer cells antigen CD94 (gene Klrd1; UniProt O54707, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.005 mg NaN3 |
| Calculated MW | 20.8 kDa |
| Observed MW | 30 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat blood, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Klrd1/CD94 fragment (Asn33–Ile179), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places natural killer cells antigen CD94 in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
