| Field | Specification |
|---|---|
| Target | |
| Alternative names | Killer cell lectin-like receptor subfamily G member 1; C-type lectin domain family 15 member A; ITIM-containing receptor MAFA-L; MAFA-like receptor; Mast cell function-associated antigen; KLRG1; CLEC15A; MAFA; MAFAL |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KLRG1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23K64 is a rabbit monoclonal antibody that recognizes killer cell lectin-like receptor subfamily G member 1 (gene KLRG1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 21.8 kDa, and the supplier reports an observed band at about 38 kDa.
KLRG1 is an inhibitory C-type lectin-like receptor expressed on natural killer cells and antigen-experienced T cells. It binds classical cadherins such as E-cadherin and sends inhibitory signals that restrain cell activity. KLRG1 is commonly used as a marker of terminally differentiated effector T cells and mature NK cells.
| Target | Killer cell lectin-like receptor subfamily G member 1 (gene KLRG1; UniProt Q96E93, human) |
|---|---|
| Clone | 23K64 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 21.8 kDa |
| Observed MW | 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human Jurkat cells, human Raji cells, human HeLa cells, human HEL cells, rat spleen tissue, rat lung tissue, mouse spleen tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human KLRG1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places killer cell lectin-like receptor subfamily G member 1 in the cell membrane. Tissue expression noted by UniProt (human): Expressed specifically on natural killer (NK) cells and T-cells, mainly CD8 T-cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
