| Field | Specification |
|---|---|
| Target | |
| Alternative names | N-lysine methyltransferase KMT5A; H4-K20-HMTase KMT5A; Histone-lysine N-methyltransferase KMT5A; Lysine N-methyltransferase 5A; Lysine-specific methylase 5A; PR/SET domain-containing protein 07; PR-Set7; PR/SET07; SET domain-containing protein 8; KMT5A; PRSET7; SET07; SET8; SETD8 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KMT5A / SETD8 / Pr-SET7 Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1) . H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADHO-11 is a rabbit monoclonal antibody that recognizes N-lysine methyltransferase KMT5A in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 42.9 kDa, and the supplier reports an observed band at about 50 kDa.
KMT5A (SETD8, PR-Set7) is the only enzyme known to add a single methyl group to histone H4 at Lys20 (H4K20me1). Its levels change through the cell cycle, and it is needed for chromatin compaction in mitosis, DNA replication control and genome stability. It also methylates non-histone proteins such as p53 and is studied in cell cycle and cancer research.
| Target | N-lysine methyltransferase KMT5A (gene KMT5A; UniProt Q9NQR1, human) |
|---|---|
| Clone | ADHO-11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.9 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): 293T cells, NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human KMT5A / SETD8 / Pr-SET7 Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places N-lysine methyltransferase KMT5A in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
