| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Histone-lysine N-methyltransferase KMT5B; Lysine N-methyltransferase 5B; Lysine-specific methyltransferase 5B; Suppressor of variegation 4-20 homolog 1; Su(var)4-20 homolog 1; Suv4-20h1; [histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B; 2.1.1.362; [histone H4]-lysine20 N-methyltransferase KMT5B; 2.1.1.361; KMT5B; SUV420H1; CGI-85 |
| Clonality | |
| Form | Liquid |
| Host | |
| Immunogen | E. coli - derived recombinant human KMT5B (Met72-Ser368). |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Overview
Anti-KMT5B Polyclonal Antibody targets KMT5B ([histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B). Frequently used across ELISA, IHC, WB workflows, depending on experimental design and sample type. This antibody is suited for workflows commonly used in Gene Expression & Epigenetics research.
Also known as: [histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B, [histone H4]-lysine20 N-methyltransferase KMT5B, Histone-lysine N-methyltransferase KMT5B, Lysine-specific methyltransferase 5B, Suppressor of variegation 4-20 homolog 1, Lysine N-methyltransferase 5B, Su(var)4-20 homolog 1, Suv4-20h1, CGI-85, 2.1.1.362.
Target biology
KMT5B ([histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B) is a modification-specific target used to assess protein regulatory state rather than total protein abundance. Post-translational modification epitopes can be stimulation- and fixation-sensitive; rapid sample processing and appropriate inhibitors can help preserve signal. Common alternative names and symbols used in the literature include: [histone H4]-lysine20 N-methyltransferase KMT5B, Histone-lysine N-methyltransferase KMT5B, Lysine-specific methyltransferase 5B, Suppressor of variegation 4-20 homolog 1, Lysine N-methyltransferase 5B, Su(var)4-20 homolog 1, Suv4-20h1, CGI-85, 2.1.1.362, 2.1.1.361. Based on the annotated name/class, the target is most often associated with nucleus localization, though this can vary by model system.
How this antibody helps
As a polyclonal reagent, this antibody can provide strong sensitivity through recognition of multiple epitopes on the target. For best performance, interpret signal in the context of appropriate biological controls and orthogonal readouts when available.
Antigen / immunogen context
E. coli - derived recombinant human KMT5B (Met72-Ser368).
Recommended experimental notes
- Titrate the antibody to optimize signal-to-noise for your specific sample type and detection system.
- Use appropriate positive and negative controls (e.g., target-high vs target-low samples, KO/KD where available).
- For Western blotting, use fresh protease (and phosphatase, if relevant) inhibitors and confirm the primary band at the expected molecular weight; additional bands may reflect isoforms or processing.
- For IHC, optimize fixation and antigen retrieval (buffer/pH/heat) and include an isotype/secondary-only control to assess background.
- For ELISA, optimize capture/detection pairing and run a standard curve in matrix-matched diluent when possible.
Additional notes
For research use only
For research use only. Not for diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
