| Field | Specification |
|---|---|
| Target | |
| Alternative names | Importin subunit alpha-1; Karyopherin subunit alpha-2; RAG cohort protein 1; SRP1-alpha; KPNA2; RCH1; SRP1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KPNA2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEAF-11 is a rabbit monoclonal antibody that recognizes importin subunit alpha-1 (gene KPNA2) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 57.9 kDa, and the supplier reports an observed band at about 58 kDa.
Importin α1 (KPNA2) is an adapter that binds classical nuclear localization signals on cargo proteins and links them to importin β1 for transport through the nuclear pore, with RanGTP triggering release inside the nucleus. KPNA2 is highly expressed in proliferating cells and is often elevated in tumors, making it a subject of cancer research.
| Target | Importin subunit alpha-1 (gene KPNA2; UniProt P52292, human) |
|---|---|
| Clone | AEAF-11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.9 kDa |
| Observed MW | 58 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells, C6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human KPNA2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places importin subunit alpha-1 in the cytoplasm, nucleus and Golgi apparatus membrane. Tissue expression noted by UniProt (human): Expressed ubiquitously. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
