| Field | Specification |
|---|---|
| Target | |
| Alternative names | Krev interaction trapped protein 1;Krev interaction trapped 1;Cerebral cavernous malformations 1 protein;KRIT1;CCM1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human KRIT1, different from the related mouse sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes krev interaction trapped protein 1 (gene KRIT1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 84.3 kDa, and the supplier reports an observed band at about 84 kDa.
KRIT1 (also called CCM1) is a scaffolding protein that, with CCM2 and CCM3, maintains the integrity of blood vessels. It supports endothelial cell–cell junctions and restrains angiogenesis, partly by limiting RhoA/ROCK signaling and regulating Notch signaling. Loss-of-function mutations in KRIT1 cause cerebral cavernous malformations, clusters of fragile, leaky vessels in the brain.
| Target | Krev interaction trapped protein 1 (gene KRIT1; UniProt O00522, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 84.3 kDa |
| Observed MW | 84 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat cardiac muscle tissue, NIH3T3 cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human KRIT1. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places krev interaction trapped protein 1 in the cytoplasm, cell membrane and cell junction. Tissue expression noted by UniProt (human): Low levels in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
