| Field | Specification |
|---|---|
| Target | |
| Alternative names | Kinase suppressor of Ras 1; KSR1; KSR |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KSR1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEOO-11 is a rabbit monoclonal antibody that recognizes kinase suppressor of Ras 1 (gene KSR1) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 102.2 kDa, and the supplier reports an observed band at about 120 kDa.
Kinase suppressor of Ras 1 (KSR1) is a scaffold protein for the ERK/MAPK cascade. It brings RAF, MEK and ERK together at the membrane and, when bound to MEK, can dimerize with BRAF to promote MEK phosphorylation. This helps set the strength and location of Ras–RAF–MEK–ERK signaling, and KSR1 is studied in growth factor signaling and Ras-driven cancer.
| Target | Kinase suppressor of Ras 1 (gene KSR1; UniProt Q8IVT5, human) |
|---|---|
| Clone | AEOO-11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 102.2 kDa |
| Observed MW | 120 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): HEK293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human KSR1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places kinase suppressor of Ras 1 in the cytoplasm, membrane, cell membrane and cell projection. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
