| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lethal; 3; malignant brain tumor-like protein 3; H-l(3)mbt-like protein 3; L(3)mbt-like protein 3; L3mbt-like 3; MBT-1; L3MBTL3; KIAA1798; MBT1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human L3MBTL3 recombinant protein (Position: Q11-R686). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects lethal(3)malignant brain tumor-like protein 3 (gene L3MBTL3) in human samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 88.3 kDa, and the supplier reports an observed band at about 90 kDa.
L3MBTL3 is a methyl-lysine reader of the MBT domain family that acts as a transcriptional corepressor. It helps RBPJ silence Notch target genes by bringing the demethylase KDM1A to Notch-responsive regions, and it also links methylated non-histone proteins such as SOX2, DNMT1 and E2F1 to the CRL4-DCAF5 ubiquitin ligase for degradation, making it of interest in chromatin and Notch signaling research.
| Target | Lethal(3)malignant brain tumor-like protein 3 (gene L3MBTL3; UniProt Q96JM7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 88.3 kDa |
| Observed MW | 90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human HEL cells, human 293T cells, human THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human bladder urothelial carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): U251 cells.
Immunogen
Recombinant human L3MBTL3 fragment (Gln11–Arg686), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places lethal(3)malignant brain tumor-like protein 3 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
